Xenbase Image ID: 77783

Fig. S3. Effects of Dlx3 or GATA2 loss of function after injection of engrailed repressor constructs on neural and non-neural ectodermal markers. Neural plate stage embryos after unilateral injection (lower half; marked by light blue β-galactosidase or green mycGFP staining) of various constructs as indicated. Reductions (arrows), and broadening or ectopic expression domains (asterisks) in the neural (green) and non-neural ectoderm (orange) compared with the control side (arrowheads) are indicated. Double asterisks indicate lateral displacement owing to widening of the neural plate. See text for details. Image published in: Pieper M et al. (2012) Copyright © 2012. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue sox3.S Sry, Xlsox3, xSox3, Xtsox3 X. laevis Throughout NF stage 18 neural plate pre-chordal neural plate chordal neural plate neural plate border anterior placodal area zic1.S odd-paired-like, opl, Zic-r1, Zic-related-1 X. laevis Throughout NF stage 18 neural plate border pre-chordal neural plate border chordal neural plate border posterior placodal area msx1.L hox-7, hox7, msx-1, msx1-a, msx1-b, Xhox-7.1, Xmsx-1, xmsx1 X. laevis Throughout NF stage 18 neural plate border pre-chordal neural plate border chordal neural plate border neural plate posterior placodal area presumptive midbrain-hindbrain boundary pax3.S cdhs, hup2, pax-3, pax3-a, pax3-b, Waardenburg syndrome 1, ws1, xpax3 X. laevis Throughout NF stage 18 chordal neural plate neural plate border pre-chordal neural plate border chordal neural plate border profundal placode foxd3.L fkh6, foxd3-a, foxd3-b, XFD-6, xfoxd3 X. laevis Throughout NF stage 18 cranial neural crest sox9.S cmd1, cmpd1, sox-9, sox9-a, sox9-b, sra1 X. laevis Throughout NF stage 18 cranial neural crest posterior placodal area epibranchial placode six1.L XSix1 X. laevis Throughout NF stage 18 anterior placodal area posterior placodal area eya1.L eyes absent-1, XEya1 X. laevis Throughout NF stage 18 anterior placodal area posterior placodal area foxi2.S foxi2-a, foxi2-b X. laevis Throughout NF stage 18 anterior placodal area dlx5.L dll3, X-dll3 X. laevis Throughout NF stage 18 non-neural ectoderm neural plate border pre-chordal neural plate border chordal neural plate border gata2.L GATA-2, xGATA-2, xgata2 X. laevis Throughout NF stage 18 non-neural ectoderm dlx3.S ai4, dlx3-a, dlx3-b, tdo, Xdll-2, xdlx3 X. laevis Throughout NF stage 18 non-neural ectoderm neural plate border pre-chordal neural plate border chordal neural plate border krt12.4.L ck81, cytok, cytokeratin, E-keratin, epi-keratin, epidermal keratin, Epidermis-specific keratin, EpiK, epiker, EpK, epK, K81, xK81, xk81a, xk81a1 X. laevis Throughout NF stage 18 non-neural ectoderm epidermis Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + dlx3del-EnR + NF16 (in situ hybridization) fig.S3.a Expression Phenotype increased amount sox3.L expression in neuroectoderm mislocalised sox3.L expression in non-neural ectoderm increased amount sox3.L expression in non-neural ectoderm Xla Wt + gata2del-EnR + NF16 (in situ hybridization) fig.S3.a^1 Expression Phenotype increased amount sox3.L expression in neuroectoderm increased amount sox3.L expression in non-neural ectoderm mislocalised sox3.L expression in non-neural ectoderm Xla Wt + dlx3del-EnR + NF16 (in situ hybridization) fig.S3.b Expression Phenotype increased amount zic1.S expression in neuroectoderm mislocalised zic1.S expression in non-neural ectoderm increased amount zic1.S expression in non-neural ectoderm Xla Wt + gata2del-EnR + NF16 (in situ hybridization) fig.S3.b^1 Expression Phenotype increased amount zic1.S expression in neuroectoderm mislocalised zic1.S expression in non-neural ectoderm increased amount zic1.S expression in non-neural ectoderm Xla Wt + dlx3del-EnR + NF16 (in situ hybridization) fig.S3.c Anatomical Phenotype increased width of neural plate Expression Phenotype decreased amount msx1.L expression in neuroectoderm Xla Wt + gata2del-EnR + NF16 (in situ hybridization) fig.S3.c^1 Expression Phenotype mislocalised msx1.L expression in neuroectoderm increased amount msx1.L expression in neuroectoderm Xla Wt + dlx3del-EnR + NF16 (in situ hybridization) fig.S3.d Expression Phenotype mislocalised pax3.S expression in neuroectoderm increased amount pax3.S expression in neuroectoderm decreased amount pax3.S expression in non-neural ectoderm Xla Wt + gata2del-EnR + NF16 (in situ hybridization) fig.S3.d^1 Expression Phenotype mislocalised pax3.S expression in neuroectoderm increased amount pax3.S expression in neuroectoderm decreased amount pax3.S expression in non-neural ectoderm Xla Wt + dlx3del-EnR + NF16 (in situ hybridization) fig.S3.e Expression Phenotype decreased amount foxd3.L expression in neuroectoderm Xla Wt + gata2del-EnR + NF16 (in situ hybridization) fig.S3.e^1 Anatomical Phenotype increased width of neural plate Expression Phenotype decreased amount foxd3.L expression in neuroectoderm Xla Wt + dlx3del-EnR + NF16 (in situ hybridization) fig.S3.f Expression Phenotype decreased amount sox9.L expression in neuroectoderm decreased amount sox9.L expression in non-neural ectoderm Xla Wt + gata2del-EnR + NF16 (in situ hybridization) fig.S3.f^1 Anatomical Phenotype increased width of neural plate Expression Phenotype increased amount sox9.L expression in neuroectoderm mislocalised sox9.L expression in neuroectoderm decreased amount sox9.L expression in non-neural ectoderm Xla Wt + dlx3del-EnR + NF16 (in situ hybridization) fig.S3.g Expression Phenotype decreased amount tubb2b.S expression in neuroectoderm decreased amount tubb2b.S expression in non-neural ectoderm Xla Wt + gata2del-EnR + NF16 (in situ hybridization) fig.S3.g^1 Expression Phenotype decreased amount tubb2b.S expression in non-neural ectoderm Xla Wt + dlx3del-EnR + NF16 (in situ hybridization) fig.S3.h Expression Phenotype decreased amount six1.L expression in non-neural ectoderm Xla Wt + gata2del-EnR + NF16 (in situ hybridization) fig.S3.h^1 Expression Phenotype decreased amount six1.L expression in non-neural ectoderm Xla Wt + dlx3del-EnR + NF16 (in situ hybridization) fig.S3.i Expression Phenotype decreased amount eya1.L expression in non-neural ectoderm Xla Wt + gata2del-EnR + NF16 (in situ hybridization) fig.S3.i^1 Expression Phenotype decreased amount eya1.L expression in non-neural ectoderm Xla Wt + dlx3del-EnR + NF16 (in situ hybridization) fig.S3.j Expression Phenotype decreased amount foxi1.L expression in non-neural ectoderm Xla Wt + gata2del-EnR + NF16 (in situ hybridization) fig.S3.j^1 Expression Phenotype decreased amount foxi1.L expression in non-neural ectoderm Xla Wt + dlx3del-EnR + NF16 (in situ hybridization) fig.S3.k Expression Phenotype decreased amount dlx5.L expression in non-neural ectoderm Xla Wt + gata2del-EnR + NF16 (in situ hybridization) fig.S3.k^1 Expression Phenotype decreased amount dlx5.L expression in non-neural ectoderm Xla Wt + dlx3del-EnR + NF16 (in situ hybridization) fig.S3.l Expression Phenotype decreased amount gata2.L expression in non-neural ectoderm Xla Wt + gata2del-EnR + NF16 (in situ hybridization) fig.S3.m Expression Phenotype decreased amount dlx3.L expression in non-neural ectoderm Xla Wt + dlx3del-EnR + NF16 (in situ hybridization) fig.S3.n Expression Phenotype decreased amount krt70.S expression in non-neural ectoderm Xla Wt + gata2del-EnR + NF16 (in situ hybridization) fig.S3.n^1 Expression Phenotype decreased amount krt70.S expression in non-neural ectoderm Larger Image Printer Friendly View

Return to previous page