Xenbase Image ID: 77795

Fig. 8. X-Delta-2 MO effect on upstream regulators of Hox genes. Non-injected controls (Nic; A,C,E,G) and embryos injected on the left-hand side with X-Delta-2 MO (B,D,F,H) were analysed at gastrula stage for the expression of Xbra (A,B: 89%, n=27) and BMP-4 (C,D: 68%, n=31), and at neurula stage for the expression of Xcad-3 (E,F: 75%, n=28) and FGF-4 (G,H: 90%, n=32) by whole-mount in situ hybridisation. X-Delta-2 MO downregulates the expression of BMP-4 and Xcad-3, without affecting the expression of Xbra and FGF-4. Embryos at gastrula stage are shown from the vegetal side, dorsal up (A–D), and at neurula stage from the dorsal side, anterior up (E,F) or posterior side, dorsal up (G,H). The arrowhead indicates the injected side of the embryo. Image published in: Peres JN et al. (2006) Copyright © 2006. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue tbxt.S bra, brachyury, ntl, t, t-a, t-b, X-bra, Xbra, Xbrachyury X. laevis Throughout NF stage 11 circumblastoporal collar involuted dorsal mesoderm marginal zone ventral marginal zone ventro-lateral marginal zone dorso-lateral marginal zone dorsal marginal zone bmp4.L bmp-4, bmp2b, bmp2b1, bmp4-a, bmp4-b, DVR-4, ofc11, XBMP-4, xbmp4, zyme X. laevis Throughout NF stage 11 involuted ventral mesoderm cdx4.L cad3, MGC130769, MGC68909, Xcad-3, Xcad3, Xtcad3 X. laevis Throughout NF stage 20 posterior neural tube fgf4.L efgf, fgf-4, fgf4-a, fgf4-b, fgfe, HBGF-4, XEFGF, Xfgf-4, Xfgf4 X. laevis Throughout NF stage 20 circumblastoporal collar paraxial mesoderm involuted dorsal mesoderm Image source: Published Larger Image Printer Friendly View

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