Xenbase Image ID: 77809

Fig. S1. The hyperpolarized cells of the anterior neural field possibly contribute to eye formation. (A) CC2-DMPE staining showing the hyperpolarized cells in the anterior neural field (red arrowhead). (B) Photoconversion from green to red florescence centered on the hyperpolarization signal using a 40lens as per the protocol (Wacker et al., 2007). (C) At stage 28 the contribution of the photoconverted cells to eye formation was observed (yellow arrowhead). Note that the photoconverted area also contributes to some regions of the forebrain, similar to Pax6. (D) Stage 28 embryo showing plane of sectioning through the eye. (E) Confocal image of a section through a stage 28 photoconverted eye, showing labeling of the majority of the lens and retinal tissue. (F) Dark-field image of the section through the eye. See also Fig. 1. Illustrations reproduced with permission from Nieuwkoop and Faber (Nieuwkoop and Faber, 1967). Image published in: Pai VP et al. (2012) Copyright © 2012. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

Return to previous page