Xenbase Image ID: 77811

Fig. S3. Hyperpolarization patterns and ectopic eye tissue induction. (A) CC2-DMPE staining of stage 19 embryos. (i) Illustration of stage 19 Xenopus embryo.. (ii) Control (uninjected) embryos show characteristic bilateral hyperpolarized cell clusters (red arrowheads). (iii) DNKir6.1p mRNA-injected (two dorsal cells at the four-cell stage) embryos show disrupted or lost hyperpolarization signal (57%, n=7). (B) Stage 42 tadpoles microinjected with DNKir6.1p mRNA in all four cells at the four-cell stage show (i,ii) ectopic eye tissue (red arrows) like patches of RPE with lens-like tissue having lateral pigmented connections to brain. (iii) β-gal signal in embryos co-injected with lacZ and the ion channel construct, showing that ectopic eye tissues arise in the regions in which Vmem was modulated. See also Fig. 3. Illustrations reproduced with permission from Nieuwkoop and Faber (Nieuwkoop and Faber, 1967). Image published in: Pai VP et al. (2012) Copyright © 2012. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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