Xenbase Image ID: 77849

Fig. 3. Geminin suppresses mesoderm and non-neural ectoderm formation. Top: downregulated genes from microarrays were assayed by qRT-PCR for expression changes upon Geminin overexpression or knockdown in embryos (left) or stage 12 ectodermal explants (center/right). Right panel compares effects of plasmid-driven (125 pg) versus mRNA-based overexpression of full-length-FL/30 pg or δcoil/300 pg Geminin on gene expression. Relative expression normalized to EF1α is shown, control (uninjected embryo/explant) values=1.0. A representative result from at least three independent experiments is shown. Bottom: confirmation of Geminin-downregulated genes by in situ hybridization. Injected region is marked by Red-gal (Grhl1/Zic1/Zic3) or X-gal staining and yellow arrowheads. For Foxi1/Grhl1/Zic1/Zic3, stage 13/neural plate view is shown with dorsal midlines indicated (lines). Image published in: Lim JW et al. (2011) Copyright © 2011. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue ventx1.2.S vent-1, vent1, Xvent-1, Xvent1 X. laevis Throughout NF stage 12 involuted ventral mesoderm posterior ventx2.2.L vent-2, vent2, Xom, xvent-2, xvent2 X. laevis Throughout NF stage 12 animal cap ventral marginal zone ventro-lateral marginal zone marginal zone ventral szl.L frzb3, secreted frizzled, sizzled, xszl X. laevis Throughout NF stage 12 ventral marginal zone ventral sall1.S LOC108715370, sal-1, sal-like 1, sal1, sall1-a, sall1-b, sall1b, spalt-like 1, tbs, znf794 X. laevis Throughout NF stage 12 marginal zone blastopore lip foxi1.L ectodermally-expressed mesendoderm antagonist, ema, FoxI1e, foxi1e, foxi3, Xema, xfoxi1 X. laevis Throughout NF stage 13 ectoderm grhl1.L grainyhead 1, lbp32, mgr, nh32, tfcp2l2, XGrhl1 X. laevis Throughout NF stage 13 ectoderm zic1.S odd-paired-like, opl, Zic-r1, Zic-related-1 X. laevis Throughout NF stage 13 ectoderm zic3.S xzic3, Zinc finger protein of the cerebellum 3 X. laevis Throughout NF stage 13 neuroectoderm Image source: Published Larger Image Printer Friendly View

Return to previous page