XB-IMG-77868
Xenbase Image ID: 77868
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Fig. S1. Microarray analysis. (A) Frontal view of embryos labeled by in situ hybridization for genes indicated. Arrows indicate the presumptive primary mouth. cg, cement gland. Scale bars: 200 . cDNAs used for making in situ probes are vgl-2 (GA# BC056001, image clone 4930090, Open BioSystems), CPN (GA# BC059995, image clone 4030455, Open Biosystems), pitx-3 GA# AF297713.1, provided by Hollemann (Pommereit et al., 2001) and six-1 GA# AF279254, provided by Moody (Pandur and Moody, 2000). (B) Expression levels of genes from Table S1, as determined by microarray (M) and qRT-PCR (R). Shading and colors match scheme shown in Fig. 1, in which the anterior dorsal region (AD) is shaded dark gray, the cement gland and ventral region (CG+V) light gray, and the presumptive primary mouth (PMo) is colored red. (C) Expression of Wnt pathway components in the primary mouth (red) relative to the anterior dorsal region (AD; dark gray), and a ventral region, including the ventral half of the cement gland (CG+V; light gray) as determined by microarray. The expression level of each gene was plotted relative to each other so that a single bar represents the total expression in all three regions, scaled to 100. In the primary mouth anlage expression of wnt-8, wnt-8b, wnt-3a and wnt-2 is significantly depleted (<6% relative to the flanking regions) and expression of wnt-4, β-catenin, axin and the Frizzled receptors frz-3 and frz-9 are also depleted (<20% relative to the flanking regions). Other Wnt inhibitors, dkk-1 and wif-1, are enriched (>57% relative to the flanking regions). Image published in: Dickinson AJ and Sive HL (2009) Copyright © 2009. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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