Xenbase Image ID: 77878
Fig. 1. osr1 and osr2 are dynamically expressed in the developing Xenopus foregut and are required for lung development. (A-G′′) In situ hybridization of osr1 (A-D) and osr2 (E-H) expression in Xenopus tropicalis embryos and isolated gut tubes (D,H) at the indicated stages (st), anterior leftwards, dorsal upwards. Stage 15 embryos (A,E) are bisected and stage 33 embryos (C′-C[H11630],G′-G[H11630]) are sectioned to show the endoderm (outlined in yellow in A,E). fg, foregut; hg, hindgut; de, dorsal endoderm; pe, posterior endoderm; e, esophagus; l/t, presumptive lung and trachea; lv, liver; lpm, lateral plate mesoderm; k, kidney; s, stomach. (I-N′) surfactant protein c (sftpc) (I-L) and nkx2.1 (M,N′) expression in embryos injected on one side with Osr1/Osr2-MOs (10 ng each) or negative control mutated oligos MM-MO (20 ng) shows lung agenesis on the Osr1/Osr2-depleted side. (M’-N′) Sections. Numbers of embryos with the shown phenotype are indicated. Dorsal view of an isolated gut tube (K,L) shows the lung bud absent on the injected side (red arrow).
Image published in: Rankin SA et al. (2012)
Copyright © 2012. Image reproduced on Xenbase with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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