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XB-IMG-77878

Xenbase Image ID: 77878


Fig. 1. osr1 and osr2 are dynamically expressed in the developing Xenopus foregut and are required for lung development. (A-G′′) In situ hybridization of osr1 (A-D) and osr2 (E-H) expression in Xenopus tropicalis embryos and isolated gut tubes (D,H) at the indicated stages (st), anterior leftwards, dorsal upwards. Stage 15 embryos (A,E) are bisected and stage 33 embryos (C′-C[H11630],G′-G[H11630]) are sectioned to show the endoderm (outlined in yellow in A,E). fg, foregut; hg, hindgut; de, dorsal endoderm; pe, posterior endoderm; e, esophagus; l/t, presumptive lung and trachea; lv, liver; lpm, lateral plate mesoderm; k, kidney; s, stomach. (I-N′) surfactant protein c (sftpc) (I-L) and nkx2.1 (M,N′) expression in embryos injected on one side with Osr1/Osr2-MOs (10 ng each) or negative control mutated oligos MM-MO (20 ng) shows lung agenesis on the Osr1/Osr2-depleted side. (M’-N′) Sections. Numbers of embryos with the shown phenotype are indicated. Dorsal view of an isolated gut tube (K,L) shows the lung bud absent on the injected side (red arrow).

Image published in: Rankin SA et al. (2012)

Copyright © 2012. Image reproduced on Xenbase with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.

GeneCloneSynonymsSpeciesStage(s)Tissue
osr1 odd1X.tropicalisThroughout NF stage 15endoderm
central endoderm
osr1 odd1X.tropicalisThroughout NF stage 28endoderm
foregut
lateral plate mesoderm
alimentary system
stomodeal-hypophyseal primordium
hindgut
intermediate mesoderm
pronephric mesenchyme
lung primordium
osr1 odd1X.tropicalisThroughout NF stage 33 and 34lung
endoderm
foregut
lateral plate mesoderm
alimentary system
anterior dorsal lateral plate region
otic vesicle
eye
mouth primordium
brain
midbrain
hindbrain
spinal cord
osr1 odd1X.tropicalisThroughout NF stage 42lung
esophagus
foregut
stomach
alimentary system
osr2 xosr2, osr2-b, osr2-aX.tropicalisThroughout NF stage 15dorsal
endoderm
central endoderm
osr2 xosr2, osr2-b, osr2-aX.tropicalisThroughout NF stage 28endoderm
lateral plate mesoderm
anterior dorsal lateral plate region
eye
foregut
intermediate mesoderm
lung primordium
dorsal lateral plate mesoderm
hindgut
osr2 xosr2, osr2-b, osr2-aX.tropicalisThroughout NF stage 42lung
esophagus
alimentary system
foregut
osr2 xosr2, osr2-b, osr2-aX.tropicalisThroughout NF stage 33 and 34endoderm
esophagus
lateral plate mesoderm
alimentary system
pronephric kidney
pronephric duct
eye
foregut
intermediate mesoderm
lung primordium
otic vesicle
spinal cord
pharyngeal region
head
brain
sftpc SP-C, xSP-C, SPC, surfcX.tropicalisSometime during NF stage 37 and 38 to NF stage 42lung
endoderm
foregut
nkx2-1.L ttf1, nkx2.1, titf1, Xnkx-2.1, xnkx2-1X.laevisThroughout NF stage 42lung
trachea
endoderm
foregut

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