Xenbase Image ID: 77879
Fig. 2. Osr1/Osr2 are required for respiratory specification and tracheal-esophageal separation. (A-L′) Embryos were co-injected with control MM-MOs or Osr1/Osr2-MOs and GR-mOsr1 RNA (250 pg) in both dorsal-vegetal blastomeres at the eight-cell stage (bilateral injection) and then cultured ±dexamethasone from stage 20 onwards. (A-C,G-I) Analysis by in situ hybridization of nkx2.1 at stage 34/35 (A-C) and sftpc at stage 42 (G-I). (D-F′,J-L′) Confocal immunofluorescence of Nkx2.1 (green) and Sox2 (red) at stage 35/36 (D-F) and stage 42 (J-L). es, esophagus; tr, trachea; lb, lung buds; st, stomach, Scale bars: 100 μm. [Curators Note: all embryos X. laevis]
Image published in: Rankin SA et al. (2012)
Copyright © 2012. Image reproduced on Xenbase with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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