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Fig. 3. Osr1/Osr2 are required for wnt2b and raldh2 expression in the lpm. (A-X) Analysis of stage 34/35 unilateral-injected embryos shows that Osr1/Osr2 are required for expression of nkx2.1 (A-C) and sox2 (D-F) in the epithelium, whereas shh (G-I) is unaffected. In the lpm, wnt2b (J-L) and raldh2 (M-O) are absent, while foxf1 is unaffected (P-R). Black arrows indicate normal expression on the control side and red arrows indicate lack of expression on the Osr1/Osr2-MO-injected side (right). Endoderm outlined by a yellow line. Numbers of embryos with the shown phenotype are indicated. fg, foregut; hg, hindgut; de, dorsal endoderm; pe, posterior endoderm; e, esophagus; l/t, presumptive lung and trachea; lv, liver; lpm, lateral plate mesoderm; k, kidney; s, stomach. Image published in: Rankin SA et al. (2012) Copyright © 2012. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue nkx2-1.L nkx2.1, titf1, ttf1, Xnkx-2.1, xnkx2-1 X. laevis Sometime during NF stage 33 and 34 to NF stage 35 and 36 lung endoderm brain forebrain eye lung primordium trachea foregut thyroid primordium sox2.L anop3, LOC108718087, mcops3, Sox-2, XLSOX-2, Xsox-2, XSox2 X. laevis Sometime during NF stage 33 and 34 to NF stage 35 and 36 stomach brain spinal cord foregut endoderm head pharyngeal arch otic vesicle esophagus eye shh.L hedgehog, vhh-1, X-SHH, Xhh, xshh X. laevis Sometime during NF stage 33 and 34 to NF stage 35 and 36 endoderm trachea esophagus foregut gall bladder primordium pharyngeal arch mandibular arch hyoid arch branchial arch head wnt2b.L wnt-2b, wnt2b-a, wnt2b-b, Xwnt-2b, xwnt2b X. laevis Sometime during NF stage 33 and 34 to NF stage 35 and 36 lateral plate mesoderm lens placode otic vesicle pineal gland pharyngeal arch aldh1a2.L raldh-2, raldh2, XRaldh2 X. laevis Sometime during NF stage 33 and 34 to NF stage 35 and 36 lateral plate mesoderm brain lens otic vesicle periocular region pronephric kidney pronephric duct adenohypophyseal placode midbrain hindbrain foxf1.L acdmpv, fkhl5, foxf1-a, foxf1-b, freac1, xfd-13 X. laevis Sometime during NF stage 33 and 34 to NF stage 35 and 36 lateral plate mesoderm pharyngeal arch anterior dorsal lateral plate region olfactory region head region Image source: Published Experiment + Assay Source Phenotypes and Disease Xtr Wt + osr1 MO + osr2 MO + NF42 (in situ hybridization) fig.3.b,b^1 Expression Phenotype decreased amount aldh1a2 expression in lung bud decreased amount nkx2-1 expression in lung bud decreased amount wnt2b expression in lung bud Xtr Wt + osr1 MO + osr2 MO + NF42 (in situ hybridization) fig.3.e,e^1 Expression Phenotype decreased amount sox2 expression in lung bud Larger Image Printer Friendly View

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