Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-IMG-77883

Xenbase Image ID: 77883


Fig. 6. Osr1/Osr2 promotes lung specification and growth by restricting BMP signaling. (A). Analysis of Osr1/Osr2-MO and control MM-MO stage 35 embryos indicated that expression of bmp4 and BMP-target genes msx1 and hand1 are expanded into the ‘wnt2b-domain’ of the lpm on the Osr1/Osr2-depleted side. Black arrows indicate normal expression, red arrows indicate absent expression and green arrows indicate enhanced expression. (B) Confocal immunostaining of stage 35 embryos for phospho-Smad1/5/8 (green) and fibronectin (red). Scale bars: 50 μm. White arrows in B indicate ectopic pSMAD1 expression in dorsal foregut endoderm. (C) Analysis of nxk2.1, sox2, wnt2b and raldh2 at stage 35 or sftpc at stage 41 in embryos injected with Osr1/Osr2-MOs or MM-MOs and treated at stages 25-35 with either DMSO (vehicle control) or 10 μM LDN193189. Insets show ventral views of wnt2b expression. For analysis of sftpc expression at stage 41, embryos were removed from the LDN193189 at stage 35 and further cultured in DMSO vehicle until stage 41. Black arrows indicate normal expression, red arrows indicate absent expression, green arrows indicate expanded expression and white arrows indicate rescued expression.

Image published in: Rankin SA et al. (2012)

Copyright © 2012. Image reproduced on Xenbase with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.

GeneCloneSynonymsSpeciesStage(s)Tissue
wnt2b.L Xwnt-2b, xwnt2b, wnt-2b, wnt2b-a, wnt2b-bX.laevisThroughout NF stage 35 and 36lateral plate mesoderm
lens
head
brain
pharyngeal arch
eye
otic vesicle
pineal gland
bmp4.L XBMP-4, bmp-4, xbmp4, bone morphogenetic protein-4, zyme, bmp2b, ofc11, bmp2b1, DVR-4, bmp4-a, bmp4-bX.laevisThroughout NF stage 35 and 36lateral plate mesoderm
heart
head
brain
pharyngeal arch
eye
msx1.L hox7, hox-7, msx-1, Xmsx-1, xmsx1, Xhox-7.1, msx1-a, msx1-bX.laevisThroughout NF stage 35 and 36lateral plate mesoderm
head
brain
pharyngeal arch
eye
lens
spinal cord
epidermis
cement gland
epibranchial placode
hand1.L eHand, hand-1X.laevisThroughout NF stage 35 and 36lateral plate mesoderm
heart
pharyngeal region
head
sftpc.S SP-C, xSP-C, SPC, surfcX.laevisThroughout NF stage 42lung
sox2.S XLSOX-2, Sox-2, XSox2, Xsox-2, anop3, mcops3X.laevisThroughout NF stage 35 and 36esophagus
foregut
stomach
brain
spinal cord

Image source: Published

Larger Image
Printer Friendly View

Quality:
Good quality Poor quality
comment [+]
Return to previous page

My Xenbase: [ Log-in / Register ]
version: [4.5.0]

Major funding for Xenbase is provided by the National Institute of Child Health and Human Development, grant P41 HD064556