Xenbase Image ID: 77884

Fig. 7. Osr1/Osr2 can repress bmp4 expression. (A) GR-mOsr1 RNA (750 pg) was injected into both dorsal-vegetal cells at the eight-cell stage and embryos were dexamethasone treated at stage 20 to induce the construct and analyzed at stage 34/35 for expression of the indicated genes. Insets show ventral views of wnt2b expression. (B) The X. laevis bmp4 promoter construct, showing two putative odd-skipped-binding sites. Green sequence indicates consensus odd skipped-binding site, red sequence is the exact bmp4 promoter sequence, and mutations to these sites are shown below indicated by ‘δ’ and in black. (C) RNA (750 pg) encoding Xenopus, mouse or Drosophila Osr factors was co-injected with the –2123 bmp4: luciferase reporter at the 32-cell stage and luciferase activity was assayed at stage 12. Average relative activity ±s.d. (D) Xenopus Osr1 or Osr2 RNA (750 pg) was co-injected with the wild-type bmp4 reporter or the mutated reporter constructs indicated in B, and the resulting average fold repression ±s.d., of the luciferase reporter from three independent experiments was determined. *P<0.005. Image published in: Rankin SA et al. (2012) Copyright © 2012. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue bmp4.L bmp-4, bmp2b, bmp2b1, bmp4-a, bmp4-b, DVR-4, ofc11, XBMP-4, xbmp4, zyme X. laevis Sometime during NF stage 33 and 34 to NF stage 35 and 36 brain mesoderm intermediate mesoderm lateral plate mesoderm eye heart otic vesicle splanchnic layer of lateral plate mesoderm liver diverticulum pharyngeal arch pharynx msx1.L hox-7, hox7, msx-1, msx1-a, msx1-b, Xhox-7.1, Xmsx-1, xmsx1 X. laevis Sometime during NF stage 33 and 34 to NF stage 35 and 36 lateral plate mesoderm eye mouth primordium pharynx spinal cord brain otic vesicle hand1.L eHand, hand-1 X. laevis Sometime during NF stage 33 and 34 to NF stage 35 and 36 heart lateral plate mesoderm head pharyngeal region wnt2b.L wnt-2b, wnt2b-a, wnt2b-b, Xwnt-2b, xwnt2b X. laevis Sometime during NF stage 33 and 34 to NF stage 35 and 36   nkx2-1.L nkx2.1, titf1, ttf1, Xnkx-2.1, xnkx2-1 X. laevis Sometime during NF stage 33 and 34 to NF stage 35 and 36   Image source: Published Experiment + Assay Source Phenotypes and Disease Xtr Wt + Mmu.osr1-GR + DEX + NF42 (in situ hybridization) fig.7.a Expression Phenotype decreased amount bmp4 expression in intermediate mesoderm decreased amount bmp4 expression in pharyngeal mesenchyme decreased amount hand1 expression in intermediate mesoderm decreased amount hand1 expression in pharyngeal mesenchyme decreased amount msx1 expression in head decreased amount msx1 expression in pharyngeal mesenchyme Larger Image Printer Friendly View

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