XB-IMG-77890
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Fig. 5. xSox6 is required for chondrocyte differentiation but not for neural crest development. (A,B) xSox6-MO or CoMO was injected unilaterally into the animal pole of two blastomeres (10 ng/cell) at the four-cell stage, and the embryos were fixed at stages 17 (A) and 25 (B). Whole-mount in situ hybridization was performed with xFoxD3 (A) and xTwist (B) probes. Brackets indicate the injected side. Frequency of specimen with the indicated phenotype (nearly equal expression) was CoMO in A, 5/5; xSox6-MO in A, 18/21; CoMO in B, 5/5; xSox6-MO in B, 11/11. (C) xSox6-MO or CoMO was injected into the animal pole of all blastomeres (10 ng/cell) at the four-cell stage. Embryo heads were harvested 48 and 72 hours after fertilization (stages 35/36 and 41, respectively). The expression levels of chondrocyte markers xMatn1 and xAgc1 were analyzed by qRT-PCR. Representative results from two independent experiments are shown. (D) xSox6-MO was injected unilaterally into the animal pole of two blastomeres (10 ng/cell) at the four-cell stage, and the embryos were fixed at stage 41. Whole-mount in situ hybridization was performed with xMatn1 and xAgc1 probes. Arrows indicate presumptive cartilaginous elements. Frequency of specimen with the indicated phenotype (markedly reduced expression) was Matn1, 11/12; Agc1, 10/10. Image published in: Suzuki T et al. (2012) Copyright © 2012. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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