Xenbase Image ID: 77972

Fig. 3. FoxF1 is required for smooth muscle differentiation in the gut. Immunostaining with antibodies against α-smooth muscle actin on transverse sections through the hindgut of CoMo (A) and FoxF1 knockdown (C) embryos at stage 43/44. α-smooth muscle actin is expressed in the smooth muscle layer surrounding the gut endoderm (A) but absent in the knockdown embryo (C). (B,D) Nuclear staining by Hoechst dye on the same sections shows a layer of smooth muscle cells with elongated nuclei (arrows) surrounding the gut of CoMo-injected embryos (B), but highly disorganized gut mesoderm in knockdown embryos (D). (E) Close-up view of the area boxed in (C), visualizing the endodermal yolk platelets (arrows) in the visceral cavity. Image published in: Tseng HT et al. (2004) Copyright © 2004. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue acta2.L a-sma, aat6, actsa, alpha smooth muscle actin, alpha-actin, alpha-SM-actin, ASMA, mymy5, SM actin, sm-actin, sma, SMactin X. laevis Throughout NF stage 43 to NF stage 44 alimentary system smooth muscle foregut Image source: Published Larger Image Printer Friendly View

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