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Fig. 6. Smad2 rescues mesendodermal gene expression when activated before but not after the MBT. Embryos were injected with a hormone inducible, activated form of Smad2 (GRtSmad2) at the 2-cell stage (along with FLDx as a lineage tracer) and then treated with SB5 beginning at the 4-cell stage. GR-tSmad2 was activated by addition of dexamethasone (dex) before the MBT (stage 7) or after the MBT (stage 9) and embryos were cultured until the onset of gastrulation and then collected for analysis of mesendodermal gene expression by ISH (A) or qRT-PCR (B), as described in Fig. 4. (In panel A, the insets show FLDx immunostaining to reveal the progeny of injected blastoemeres and the numbers in the upper left indicate the frequency of the displayed phenotype/expression pattern). Error bars represent standard deviation for a representative experiment. Image published in: Skirkanich J et al. (2011) Copyright © 2011. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue gsc.L goosecoid, gsc-a, gsc-b, Xgsc X. laevis Throughout NF stage 10.5 endomesoderm prechordal plate tbxt.S bra, brachyury, ntl, t, t-a, t-b, X-bra, Xbra, Xbrachyury X. laevis Throughout NF stage 10.5 marginal zone mesoderm sox17a.L sox17 alpha, sox17-alpha, Sox17alpha, tSox17alpha, xsox17, Xsox17-alpha, xsox17a, Xsox17alpha, xSox17alpha1, xSox17alpha2 X. laevis Throughout NF stage 10.5 endomesoderm endoderm vegetal endoderm central endoderm vegetal yolk mass Image source: Published Larger Image Printer Friendly View

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