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Fig. 2. Hypoxia decreases Akt phosphorylation. (A) Western blot analysis. Protein extracts at stages 21 and 25 from embryos exposed to 21%, 10% and 5% O2 were resolved by electrophoresis, blotted and reacted with anti-Thr308-Akt, anti-Ser473-Akt or anti-Akt antibodies. Anti-tubulin alpha antibodies were used as a loading control. (B-C) Densitometric quantification of Thr308-Akt (B) and Ser473-Akt (C) from immunoblots in the different experimental conditions as described in material and method. The level of phospho-Akt decreased in hypoxia. *P<0.05; **P<0.005. Image published in: Hidalgo M et al. (2012) Copyright © 2012. Image reproduced with permission of the Publisher, The Company of Biologists Ltd. Larger Image Printer Friendly View

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