Xenbase Image ID: 78252

Figure 5. Effect of inverse agonist (AGN193109) on Xenopus development and expression of anterior markers. (a) The effects of AGN193109 were tested on 10−6 M RA-treated embryos. RA treated embryos (top), 10−6M RA + 10−7 M AGN193109 (second), 10−6 M RA + 10−6 M AGN193109 (third), 10−6 M RA + 10−5 M AGN193109 (fourth). (b) The effects of AGN193109 on normal Xenopus development. AGN193109 causes enlargement of head and short trunk (top three) compared with control embryo (bottom). (c) Dorsal view of embryo treated with AGN193109 (top) and control embryo (bottom). (d) Effects of RA and AGN193109 on anterior neural markers. Embryos were treated with RA (lane 1, RA), with AGN193109 (lane 3, 109), solvent control (lane2, sol) and all were analyzed by RNase protection assay at the neural late stage (stage 20) for the expression of Xotx2, XBF1, and XANF1. Whereas RA suppressed the expression of all three markers (lane1, RA), AGN193109 enhanced expression of the same markers (lane 3, 109). Image published in: Koide T et al. (2001) Copyright © 2001. Image reproduced on with permission of the Publisher, Cold Spring Harbor Laboratory Press. This is an Open Access article. Larger Image Printer Friendly View

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