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Rescue of Slug MO effects by Bcl-xL A: Injection of the Slug MO leads to an increase in TUNEL staining. B: This increase is blocked by the injection of Bcl-xL RNA (600 pg/embryo and co-injected with LacZ RNA). Injected sides of embryos are marked by an “*” and red staining; line marks midline of the embryo, with anterior (“An”) and posterior (“Ps”) indicated. Injection of Bcl-xL RNA (600 pg/embryo) rescues Apod (C- Slug MO injected, D-Slug MO+Bcl-xl RNA injected), Xbra (ε- Slug MO injected, F-Slug MO+Bcl-xl RNA injected), and Sox9 expression (G- Slug MO injected, H-Slug MO+Bcl-xL RNA injected). I: Injection of Bcl-xL RNA into one cell of a two-cell embryo led to a dramatic increase in the intensity and extent of Slug expression at stage 16; the region of Slug expression on the uninjected (control) side of the embryo is indicated by the dashed circle. J: Injection of Bcl-xL RNA produced an increase in Slug RNA levels in animal caps prepared at stage 8/9 and analyzed by QRT-PCR when uninjected embryos reached stage 11. Ornithine decarboxylase (ODC) was used as normalization control. RNA levels in the control case were set to 100%. Image published in: Zhang C et al. (2006) Zhang et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue vegt.L antipodean, brat, tVegT, vegt-a, vegt-b, xombi X. laevis Throughout NF stage 11 involuting marginal zone mesoderm tbxt.S bra, brachyury, ntl, t, t-a, t-b, X-bra, Xbra, Xbrachyury X. laevis Throughout NF stage 11 involuting marginal zone mesoderm sox9.S cmd1, cmpd1, sox-9, sox9-a, sox9-b, sra1 X. laevis Throughout NF stage 13 neural crest otic region snai2.L slug, snai2-a, snai2-b, Snail2, xSlu, xslug, XSnail2 X. laevis Throughout NF stage 13 neural crest Image source: Published Larger Image Printer Friendly View

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