Xenbase Image ID: 78294

Figure 3. In Situ Hybridization Revealing Ex- pression of XFli-1, VEGF, and SCL in the DLP- Hypochord Region (A) Time course from stage 26 to 34 showing XFli-1 expression in the DLP-hypochord re- gion. Numbers at the top right corner indicate the stage of development. 30b shows a high magnification view of the hypochord region. Position of the notochord (n) and hypochord (black asterisk) are indicated. Red arrows in- dicate the position of the migrating XFli-1 positive cells. Green arrow in 30a points to XFli-1-positive cells migrating from the dorsal fin to the hypochord. Red arrow in 34 indicates XFli-1 cells located in the forming dorsal aorta. Black arrow in 34 indicates the position of the posterior cardinal vein. (B) Time course from stage 26 to 32 showing expression of VEGF in the hypochord. Numbers at the top right corner indicate stage of development. Position of the notochord (n) is indicated. Arrowhead indicates the hypochord. Note that VEGF expression in the hypochord decreases after stage 30, and that by stage 32, VEGF expression is no longer detected by in situ hybridization. (C) Time course from stage 26 to stage 33 showing SCL expression in the DLP-hypochord region. Numbers at the top right corner indicate the stage of development. SCL expression in the DLP increases from stage 26 to stage 30 and then fades away with time. By stage 33, SCL expression in the DLP cannot be detected by in situ hybridization. Note that no SCL-positive cells are detected between the DLP and the midline or in the surroundings of the hypochord at any time, even at stage 30 when the strongest expression of SCL in the DLP is observed. Position of the notochord (n) and hypochord (red asterisk) are indicated. Black arrows indicate the position of the posterior cardinal veins. All sections in transverse orientation. Sections were taken at the same level of the A axis. Dorsal is at the top in all cases. Image published in: Ciau-Uitz A et al. (2000) Copyright © 2000. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue fli1.S fli, fli-1, fli1-a, Xfli, Xfli-1, Xfli1 X. laevis Sometime during NF stage 26 to NF stage 27 anterior dorsal lateral plate region fli1.S fli, fli-1, fli1-a, Xfli, Xfli-1, Xfli1 X. laevis Sometime during NF stage 28 to NF stage 33 and 34 anterior dorsal lateral plate region vegfa.L vegf, vegf-a, vegfa-a, vegfa-b X. laevis Throughout NF stage 26 to NF stage 31 hypochord tal1.S bHLHa17, stem cell leukaemia, tal-1, tcl5, xscl X. laevis Throughout NF stage 26 anterior dorsal lateral plate region spinal cord tal1.S bHLHa17, stem cell leukaemia, tal-1, tcl5, xscl X. laevis Throughout NF stage 27 to NF stage 31 anterior dorsal lateral plate region Image source: Published Larger Image Printer Friendly View

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