Xenbase Image ID: 78326

Fig. 2. The 625-bp wild-type Xenopus ANF promoter fragment recapitulates the endogenous ANF expression pattern. (A) In situ hybridization analysis of stage 45 nontransgenic heart showing ANF expression throughout the atrium, ventricle, and outflow tract. (B) Stage 49 nontransgenic Xenopus heart showing restriction of ANF transcripts to the atrial myocardium. (C) Stage 44 transgenic embryo showing GFP reporter expression driven by the wild-type 625-bp ANF promoter. This is a ventrolateral view of a living embryo under combined UV and visible light. GFP fluorescence can be observed in the eye, driven from the control -crystallin promoter and throughout the heart. (D) Fluorescent image of embryo in (C), highlighting GFP reporter expression. (E) Heart of living stage 44 transgenic embryo, with GFP expression driven from the wild-type 625-bp ANF promoter. Fluorescence is visible throughout the atrium, ventricle, and outflow tract. (F) Fluorescent image of dissected stage 48 transgenic heart, showing that GFP reporter expression is now limited to the atria. (G) In situ hybridization analysis of dissected stage 45 transgenic heart, showing distribution of GFP transcripts throughout the heart. (H) In situ hybridization analysis of GFP transcripts in dissected stage 49 transgenic heart. Note restriction of transgene expression to the atria, confirming the expression pattern observed using fluorescence. Abbreviations: o, outflow tract; a, atria; v, ventricle. Image published in: Small EM and Krieg PA (2003) Copyright © 2003. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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