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Fig. 3. Mutational analysis of the Xenopus ANF promoter in transgenic embryos. The different ANF promoter constructions are illustrated at the left of the figure. Numbers on the top line indicate the distance of the regulatory element upstream of the transcription start site. The number of independently generated transgenic embryos is indicated in the first column and the relative activity of the mutant constructions (expressed to the nearest 5%) is presented in the second column. This number is calculated as the proportion of mutant transgene embryos showing visible reporter expression relative to the number of wild type transgene embryos showing reporter expression (see Materials and methods).

Image published in: Small EM and Krieg PA (2003)

Copyright © 2003. Image reproduced with permission of the Publisher, Elsevier B. V.

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