Xenbase Image ID: 78328

Fig. 4. Mutation of the NKE within the ANF promoter results in ectopic transgene expression outside of the heart. All transgenic embryos shown are mutant for the NKE, but identical results are observed for the GATAp mutant promoter. (A) Fluorescent image of a living stage 32 transgenic embryo. Ectopic expression of reporter GFP is visible in the developing facial muscles (indicated by an arrow head). Expression in the brain, from the control crystallin promoter, as indicated by an arrow. Apparent fluorescence in the flank of the embryo is background due to autofluorescence of yolk granules. Cardiac expression of the transgene has not commenced at this stage. (B) In situ hybridization detection of GFP reporter gene expression in stage 32 embryo also shows transcripts in developing facial muscles (arrowhead). (C) Combined brightfield and UV image of stage 45 transgenic embryo. Ventrolateral view shows ectopic reporter expression in facial muscles and muscles associated with the third and fourth aortic arch artery. (D) Fluorescent image of the tadpole in (C) showing ectopic GFP expression in the interbranchialis I and II (ib1 and ib2) adjacent to the third and forth aortic arch arteries, the subarcualis obliquus (so) muscle, and the orbitohyoideus (or) muscle. Expression is also observed in the kidneys (ki). Transgene expression is strong in the heart (arrow). (E) Ventral view of the head of a different transgenic embryo shows additional facial muscles expressing GFP. The orbitohyoideus (or) associated with the musculus quadratohyangularis (qha) and the musculus interhyoideus (ih) are indicated. Control -crystallin promoter-driven GFP is observed in the eye. (F) Dorsal-view of stage 45 transgenic embryo showing ectopic GFP expression in the kidneys (ki). Image published in: Small EM and Krieg PA (2003) Copyright © 2003. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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