XB-IMG-78329
Xenbase Image ID: 78329
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Fig. 5. Mutations of the NKE or proximal GATA (GATAp) element within the ANF promoter result in persistent transgene expression throughout the ventricle and
outflow tract. (A) In situ hybridization analysis of dissected nontransgenic stage 49 heart, showing restriction of endogenous ANF transcripts to the atrium. Note
patchy expression of endogenous gene. (B) Fluorescent image of GFP expression in dissected stage 49 heart transgenic for the wild-type ANF promoter. Reporter expression is restricted to atria and also shows patchy expression. (C) Fluorescent image of dissected stage 52 transgenic heart. Mutation of the NKE within the ANF promoter results in persistent expression of the GFP reporter throughout the ventricle, and outflow tract. This ectopic expression persists for at least 3 weeks after expression of endogeneous ANF, or the wild-type ANF transgene, has restricted to the atria. (D) Fluorescent image of GATAp mutant transgene expression in the heart of a living stage 50 embryo. Expression is visible throughout the ventricle and outflow tract, exactly as observed with the NKE mutant transgene. Image published in: Small EM and Krieg PA (2003) Copyright © 2003. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |