Xenbase Image ID: 78331

FIG. 2. Subcellular localization of hermes in the oocyte. (A) Hermes is present in both the nuclear and cytoplasmic compartments. Syn- thetic mRNAs encoding either hermes, the cytoplasmic protein EF-1alpha, or the nuclear protein Max were injected into the cytoplasm of Xenopus oocytes, together with [35S]methionine. Oocytes were manu- ally dissected into cytoplasmic (C) and nuclear (N) fractions. Protein extracts equivalent to one-fifth of each fraction from a single oocyte were resolved on SDS-PAGE and visualized by fluorography. Results indicate that the control proteins EF-1alpha and Max localize to the cytoplasmic and nuclear fractions, respectively. hermes is present primarily in the cytoplasm, but a small portion of hermes is found in the nuclear fraction. (B, C) Immunolocalization of epitope-tagged hermes protein in the oocyte. Synthetic mRNAs encoding myc- epitope-tagged proteins were injected into mature oocytes, and the resulting proteins were visualized by immunocytochemistry on sec- tioned oocytes. (B) View of the vegetal region of a MTHermes-injected oocyte. Staining appears as a punctate pattern. (C) View of the vegetal region of an oocyte expressing myc-epitope tag alone. Staining is diffuse and does not show the punctate pattern seen with MThermes. Image published in: Gerber WV et al. (2002) Copyright © 2002. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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