Xenbase Image ID: 78347

Figure 1. Tiki1 Promotes Anterior Development and Inhibits Wnt Signaling(A) Dorsal injection of Tiki1 mRNA (100 pg) induced enlarged head similar to that induced by Dkk1.(B) Tiki1 domain scheme. SP, signal peptide; TM, transmembrane.(C) Phylogenetic tree of Tiki proteins from human, Xenopus, zebrafish, Nematostella, C. elegans, and Amphimedon by ClustalW (see Figure S1). TIKI domain is homologous to bacterial gumN (see Figure S2A).(D) Tiki1 inhibited Xwnt8-induced Xnr3 expression in animal caps. EF-1 alpha: a loading control; uninj: uninjected embryos; WE: whole embryos.(E) Tiki1 inhibited axis duplication by Xwnt8 but not by LRP6-delta-N, Xdsh, or beta-catenin. LDLR: a control. Numbers indicate embryos scored.(F and G) Tiki1 did not inhibit Nodal or FGF signaling.See also Figures S1 and S2. Image published in: Zhang X et al. (2012) Copyright © 2012. Image reproduced with permission of the Publisher, Elsevier B. V. Experiment + Assay Source Phenotypes and Disease Xtr Wt + dkk1 + NF27 (morphology) fig.1.a Anatomical Phenotype decreased length of anterior-posterior axis increased size of the head Xtr Wt + trabd2b + NF27 (morphology) fig.1.a Anatomical Phenotype decreased length of anterior-posterior axis increased size of the head Larger Image Printer Friendly View

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