Xenbase Image ID: 78349

Figure 3. Tiki1 Is Required for Anterior Development(A) Tiki1MO (5, 10, 20, and 40 ng) inhibited protein expression from Tiki1-FLAG but not HA∗Tiki1 mRNAs (500 pg).(B) Tiki1MO (20 ng) caused anterior defects, which were rescued by HA∗Tiki1 mRNA (50 pg). Lateral (left) and dorso-anterior (right) views are shown at stage 42.(C) Statistical data of the Tiki1MO phenotype.(D) Tiki1MO suppressed head organizer gene expression at stages 10.5–11. See also Figure S3 and Tables S1, S2, and S3. Image published in: Zhang X et al. (2012) Copyright © 2012. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue chrd.S chd, chordin, chrd.1, LOC108716939, X-chordin X. laevis Sometime during NF stage 10.5 to NF stage 11 dorsal marginal zone gsc.L goosecoid, gsc-a, gsc-b, Xgsc X. laevis Throughout NF stage 10.5 to NF stage 11 dorsal marginal zone lhx1.L lim-1, Lim1, lim1, Xlim-1, Xlim1 X. laevis Throughout NF stage 10.5 to NF stage 11 dorsal marginal zone upper blastopore lip otx2.S otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 X. laevis Sometime during NF stage 10.5 to NF stage 11 dorsal marginal zone upper blastopore lip dkk1.S dickkopf-1, Dickkopf1, dkk, dkk-1, Xdkk-1, xdkk1 X. laevis Sometime during NF stage 10.5 to NF stage 11 upper blastopore lip dorsal marginal zone involuted dorsal mesoderm nodal3.1.L nr3, Xnr-3, Xnr3 X. laevis Throughout NF stage 10.5 to NF stage 11 dorsal marginal zone involuting marginal zone not.S flh, floating head, not-a, not-b, not1, noto, Xnot, Xnot-2, Xnot1, Xnot2, Znot X. laevis Sometime during NF stage 10.5 to NF stage 11 dorsal Image source: Published Experiment + Assay Source Phenotypes and Disease Xtr Wt + trabd2a MO + NF42 (morphology) fig.3.b Anatomical Phenotype abnormal midgut morphology abnormally incomplete structure of forebrain absent cement gland absent eye decreased size of the cement gland decreased size of the eye edematous ventral trunk fused eyes increased height of the dorsal-ventral axis increased size of the head Xtr Wt + trabd2a MO + NF10.5-11 (in situ hybridization) fig.3.d Expression Phenotype decreased amount chrd.1 expression in dorsal marginal zone decreased amount dkk1 expression in dorsal marginal zone decreased amount gsc expression in dorsal marginal zone decreased amount lhx1 expression in dorsal marginal zone decreased amount otx2 expression in dorsal marginal zone Larger Image Printer Friendly View

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