Xenbase Image ID: 78352

Figure 6. Wnt3a Oxidation-Oligomerization due to Tiki Cleavage(A–D) Secreted Wnt3a in CM. The arrow and ∗ indicate Wnt3a monomers and oligomers in nonreducing gels, respectively (top). Note the enormous size of the upper ∗ band. Reducing gels (bottom) were also shown.(A) Wnt3a from TIKI2-expressing cells formed oxidized oligomers exclusively.(B) Wnt3aδN formed oxidized oligomers.(C) Oxidized Wnt3a oligomers partitioned exclusively in the aqueous phase, whereas Wnt3a monomers partitioned in detergent.(D) Wnt3a(C77A) formed oxidized oligomers regardless of TIKI2. This experiment was performed together with the one presented in (A), and a common control lane was used. Secreted Wnt3a(C77A) after TIKI2 cleavage migrated slower in the reducing gel due to altered N-glycosylation (see Figure S4E).(E) Wnt3a, Wnt3a(C77A), and Wnt3a(S209A) behaved similarly in whole-cell lysates. Each existed as monomers (arrow) and heterogeneous oxidized species from dimers (arrowhead) to larger oligomers (∗), regardless of TIKI2 cleavage.See also Figures S6 and S7. Image published in: Zhang X et al. (2012) Copyright © 2012. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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