Xenbase Image ID: 78449

Fig. 4. Nuclei in the degenerating hypochord show characteristics of apoptosis. Nuclei are detected by SYTOX green staining. Highly condensed chromatin and nuclear fragmentation are evident along the hypochord of stage-41 and -42 embryos. All sections are transverse through the trunk. (A) Bright-field and (B) fluorescence views of section through the hypochord of a stage-38 embryo. An intact nucleus is evident in the hypochord in this section (white arrowhead) and nuclear morphology is similar to other embryonic cells. (C) Bright-field and (D) fluorescence views of a section through the hypochord of a stage-41 embryo. Notice the nuclear fragmentation evident in the hypochord cell nucleus (white arrowhead). (E,F) Higher magnification views of sections through the hypochord of stage-42 embryos. Note fragmented nuclei in the hypochord (white arrowheads). n, notochord; a, dorsal aorta. Image published in: Cleaver O et al. (2000) Copyright © 2000. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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