XB-IMG-78451
Xenbase Image ID: 78451
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Fig. 6. Addition of notochord tissue results in increased VEGF expression and the development of enlarged hypochords. All panels show transverse sections through embryos that have been assayed for VEGF expression by in situ hybridization. n, notochord tissue. (A) Section through an unmanipulated stage-26 embryo showing VEGF expression in the hypochord precursor cell, located in the dorsal endoderm, immediately ventral to the notochord. (B,C) Sections through embryos that received homotopic notochord transplantations at stage 14. (B) Section through a stage-26 embryo that has received a transplanted notochord. The transplanted notochord is under the neural tube to the right and the endogenous notochord is under the neural tube to the left. Notice the significantly increased VEGF staining in the endoderm ventral to the notochords (black arrowhead). (C) Section through a stage-26 embryo in which the transplanted notochord has fused with the endogenous notochord. This notochord hybrid is now twice the size of a normal notochord. Notice the extensive VEGF expression in the endoderm ventral to the notochord (black arrowhead). (D) Section through an unmanipulated stage-30 embryo showing VEGF expression in the histologically distinct hypochord, located immediately ventral to the notochord and dorsal to the endoderm. (E,F) Sections through stage-30 embryos that have received a transplanted notochord. In both sections the transplanted notochord is on the right. Significantly enlarged hypochords (indicated by black arrowheads) are distinguishable both by histological appearance and by increased VEGF staining. Image published in: Cleaver O et al. (2000) Copyright © 2000. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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