Xenbase Image ID: 78506

Fig. 7. SRF and GATA4 can simultaneously bind XMLC2 promoter in vitro, and can synergistically activate an XMLC2-luciferase reporter in animal pole explants. (A) The CArG-like site in –122/–85 promoter fragment binds SRF only weakly (complex labelled S); GATA4 protein binds efficiently to the GATA#2 site present in the same probe (labelled G); simultaneous binding of SRF and GATA4 can be detected (arrow). (B) Overexpression of SRF activates an XMLC2(–123/–41) cyt.actin-luciferase reporter only weakly in animal pole explants, while ectopic GATA4 gives strong activation. Co-expression of both factors leads to synergistic activation. Animal poles were excised at stage 9 and collected after three hours of incubation at 23°C. Firefly luciferase activity was normalised using coinjected tk-Renilla luciferase as a control and was set at 1 for XMLC2(–123/–41) cyt.actin-luciferase in the absence of SRF or GATA4. Values shown represent a single experiment; a second experiment gave similar results. Image published in: Latinkic BV et al. (2004) Copyright © 2004. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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