Xenbase Image ID: 78508

Fig. 2. Sequence of the XMLC2 promoter. (A) The XMLC2 promoter contains GATA-, MEF2- and CArG-binding motifs (shown in red, green and blue, respectively), which are potential binding sites for GATA, MEF2 and SRF transcription factors. An additional proximal motif (purple) combines a YY1 site with a weak SRF-binding site. The proximal 700 nucleotides of promoter sequence are shown, with the TATA box (bold) and 19 nucleotides of exon1 (lower case) indicated. The minimal promoter fragment capable of supporting heart-specific expression in transgenic studies (–123/–41) is highlighted. (B) Promoter regions used in transgenesis experiments. For some constructs, the promoter fragment was fused to a heterologous minimal promoter from the Xenopus laevis cytoskeletal actin or Herpes simplex thymidine kinase genes (orange box). All other constructs included to XMLC2a basal promoter and transcription start site. Image published in: Latinkic BV et al. (2004) Copyright © 2004. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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