Xenbase Image ID: 78551

Fig. 2. Maternal PP2A:B56ϵ is required for dorsal development. (A) Morpholino antisense oligonucleotide directed against PP2A:B56ϵ blocks the translation of flag-tagged PP2A:B56ϵ RNA, but not flag-tagged PP2A:B56ϵ-c, in Xenopus oocytes, as determined by western blotting. EGFP mRNA was coinjected and western blotted with anti-GFP as a control. (B) Vegetal view of an uninjected host-transfer embryo at the onset of gastrulation (stage 10+), showing formation of the dorsal lip of the blastopore. (C) Vegetal view of a maternal PP2A:B56ϵ-depleted embryo also at stage 10+, showing absence of dorsal lip. (D) RT-PCR showing the gene expression profile in maternal PP2A:B56ϵ and β-catenin-depleted embryos at midgastrula stage (stage 11). (E) RT-PCR showing reduced expression of Xnr5 and Xnr6 at the 1,000-cell stage and reduced expression of siamois and Xnr3 at gastrula stage (stage 10). (F) RT-PCR showing reduction of dorsal gene expression in maternal PP2A:B56ϵ-depleted embryos could be rescued by PP2A:B56ϵ-c mRNA. Image published in: Yang J et al. (2003) Copyright © 2003. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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