XB-IMG-78645
Xenbase Image ID: 78645
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Fig. 4. XMeis3 and RA interactions in animal cap explants. (A) One-cell stage embryos were injected in the animal hemisphere with 1.6 ng of XMeis3- antimorph (AM) encoding RNA. Eighteen animal cap explants were removed from uninjected and injected groups of blastula embryos (stage 8 – 9) and treated with RA (1.0 AM) at stage 10.25. Explants from each group were grown to stage 18 and total RNA was isolated. RT-PCR analysis was performed with the markers HoxD1, RARa2.2, HoxB1, HoxB3, and HoxB4. EF1a served as a control for quantitating RNA levels in the different samples. For controls, RT-PCR and -RT-PCR was performed on total RNA isolated from normal embryos. (B) One-cell stage embryos were injected in the animal hemisphere with 1.6 ng of XMeis3 encoding RNA. Eighteen animal cap explants were removed from uninjected and injected groups of blastula embryos (stage 8 – 9) and treated with RA (0.1 AM). Explants from each group were grown to stage 18 and total RNA was isolated. RT-PCR analysis was performed with the markers: HoxB9, Krox20, RARa2.2, and HoxD1. EF1a served as a control for quantitating RNA levels in the different samples. For controls, RT-PCR and -RT-PCR was performed on total RNA isolated from normal embryos. (C) One-cell stage embryos were injected in the animal hemisphere with either 1.6 ng of XMeis3 or XCYP26 encoding RNAs. Eighteen animal cap explants were removed from uninjected and injected groups of blastula embryos (stage 8 – 9). Explants from each group were grown to stage 18 and total RNA was isolated. RT-PCR analysis was performed with the markers: Krox20, HoxB3, and HoxD1. EF1a served as a control for quantitating RNA levels in the different samples. For controls, RT-PCR and -RT-PCR was performed on total RNA isolated from normal embryos. Image published in: Dibner C et al. (2004) Copyright © 2004. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |