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Fig. 3. Frequencies of mutations and abnormal embryos induced by the indicated TALENs or ZFNs in Xenopus. Mutation frequencies were assayed as shown in Fig. 2. Only the mutation ratios induced by lower doses of ZFNs are shown because higher doses (200, 500, and 800 pg) resulted in dead or malformed embryos. (A) Frequencies of targeted mutagenesis induced by TALENs or ZFNs for the genes and at the doses indicated in the panels. All data refer to X. tropicalis except in C where X. laevis results are also shown. (D) Percentage of normal, abnormal, and dead X. tropicalis embryos injected with the indicated doses of TALEN or ZFN mRNAs. The injected embryos were inspected at 48 h postfertilization (about stage 41). (G) Overall morphology of X. tropicalis embryos injected with TALEN mRNAs directed against the indicated gene at stage 41. Curled axis, repression of head structures including eyes, and loss of pigments were observed. Such abnormal tadpoles usually could not complete metamorphosis.

Image published in: Lei Y et al. (2012)

Copyright © 2012. Image reproduced on Xenbase with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.

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