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Xenbase Image ID: 78650

Fig. 4. Phenotypes of ptf1a/p48-TALEN targeted X. tropicalis. (A and B) Anatomical analysis revealed visceral abnormalities such as much reduced pancreas in ptf1a/p48-TALEN-injected G0 froglets. The pancreas is outlined by dashed lines. (C) DNA sequencing of genomic DNA extracted from hindlimb tissue dissected from a froglet showing a phenotype similar to that in B confirmed the gene disruption at the ptf1a/p48 locus (20/23) (du, duodenum; st, stomach). (D) Whole-mount in situ hybridization of pancreas marker pdip in X. tropicalis tadpoles injected with the indicated TALEN mRNAs. ptf1a/p48-TALENs, but not ets1-TALENs, induced repression of pancreas bud formation. (D) Uninjected control embryos, (E) ptf1a/p48-TALEN- injected embryos (800 pg), and (F) ets1-TALEN-injected embryos (800 pg). (G) Summary of the phenotypes shown in D. The TALEN mRNAs were injected into the animal pole region at the one-cell stage; embryos were analyzed at stage 40.

Image published in: Lei Y et al. (2012)

Copyright © 2012. Image reproduced on Xenbase with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.

pdia2 pdi, XPDIp, pdipX.tropicalisThroughout NF stage 40pancreas
dorsal pancreatic bud
ventral pancreatic bud

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