Xenbase Image ID: 78694

Fig. 1. hh inactivation lengthens the cell cycle and anticipates Xvsx1 and Xotx2 translation in early retina progenitor cells. (A–F) BrdU cumulative analysis of st. 33 retina. (A, B, D, and E) BrdU (green) and nuclei (Hoechst, blue) detection on retina sections. The slope of the line in C–F indicates the rate of the cell division cycles (see Materials and Methods). (n) number of scored cells. (G–J) Xotx2 and Xvsx1 immunodetection (red) and nuclear detection (Hoechst, blue) on st. 35 retina. GCL indicates ganglion cell layer, which is detectable in control retinas (G and I) but not in cyclopamine-treated retinas (H and J). (K) Cyclopamine/control relative ratio of Xvsx1 or Xotx2 mRNA as detected by qRT-PCR in st. 35 dissected retinas. Red dashed line marks ratio = 1, bars show SE. (L) Xotx2 and Xvsx1 developmental expression of mRNA (—aaa) and protein (colored item) in WT, cyclopamine-treated, and HUA-treated retinas. Image published in: Decembrini S et al. (2009) Copyright © 2009. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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