Xenbase Image ID: 78824

Fig. 5. Xiro1 controls the expression of Otx2 and Gbx2 at different developmental stages. Embryos were injected in one blastomere at two-cell stage with 2 ng of Xiro1 mRNA (A-C), 0.5 ng of HD-GR-EnR (D,G,J,M,P,S), 0.5 ng of HD-GR-E1A (E,H,K,N,Q,T) or 0.5 ng of HD-GR (F,I,L,O,R,U) and the expression of Otx2, Gbx2 and Pax2 were analyzed at early neurula stage (stage 14). Activation of the inducible constructs was achieved by adding dexamethasone at stage 9.5-10 (D-L) or at stage 12-12.5 (M-U). Embryos injected with Xiro1 mRNA show a caudal expansion of Otx2 (A, broken lines), expansion and caudal shift of Gbx2 (B, broken lines), and Pax2 is displaced caudally (C, broken lines). (D-I) Otx2 (green) and Gbx2 (purple) were expanded and shifted caudally in embryos injected with HD-GR-EnR mRNA (D,G, broken lines). HD-GR-E1A and HD-GR repressed Otx2 and Gbx2 expression when activated at stage 9.5-10 (E,H and F,I, arrowheads). A caudal shift of Pax2 expression is observed in embryos injected with HD-GR-EnR when activated at stage 9.5-10 (J, broken lines). The injection of both HD-GR-E1A and HD-GR repress Pax2 midbrain expression domain (K,L, arrowheads). (M-O) Otx2 midbrain territory is inhibited and shifted rostrally in embryos injected with HD-GR-EnR mRNA (M, broken lines). A caudal expansion in Otx2 expression is produced by HD-GR-E1A and HD-GR overexpression and activation at stage 12-12.5 (N,O, broken lines). (P-R) Gbx2 expression is expanded anteriorly in embryos injected with HD-GR-EnR mRNA and activated at stage 12-12.5 (P, broken lines), while the injection of HD-GR-E1A and HD-GR mRNAs promote repression of Gbx2 (Q,R, arrowheads). (S-U) Embryos injected with HD-GR-EnR and activated at stage 12-12.5 causes an anterior shift of Pax2 expression (S, broken lines), while HD-GR-E1A and HD-GR produce repression and caudal displacement of Pax2 expression when activated at stage 12-12.5 (T,U, broken lines). Arrowheads indicate the injected sides. Each experiment was performed at least twice with a minimum of 20 embryos. The percentage of effect for each experiment was ∼70%. Image published in: Glavic A et al. (2002) Copyright © 2002. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue otx2.S otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 X. laevis Throughout NF stage 14 forebrain anterior neural fold gbx2.2.L XGBX-2 X. laevis Throughout NF stage 14 midbrain-hindbrain boundary pax2.L LOC108697493, pax-2, pax2-a, pax2-b, XPax-2, XPax2 X. laevis Throughout NF stage 14 midbrain-hindbrain boundary Image source: Published Larger Image Printer Friendly View

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