Xenbase Image ID: 78871

Figure 3. In situ hybridization shows expression patterns of a subset of the genes used to assess cell-type enrichment by TRAP. Representative images of retina sections from wildtype stage 57 tadpoles show mRNA expression patterns for genes selectively expressed in RGCs, rods, and Müller cells (rbpms, gnat1, rlpb1, respectively) and a gene expressed widely in multiple cell types (gapdh). A composite image of the in situs (shown in top row), DAPI and brightfield is used to identify cell layers (Merge DAPI + Phase; 2nd row). RPE, retinal pigment epithelium; ONL/INL, outer and inner nuclear layers; IPL, inner plexiform layer; GCL, ganglion cell layer. Scale bar = 20 μm.Download figure to PowerPoint Image published in: Watson FL et al. (2012) Copyright © 2012. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

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