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Figure 6. TRAP provides cell-type enrichment for genes of different absolute expression levels and reproducibility across different transgenic lines in the X. laevis retina. A: PCA of individual samples showing qPCR-based expression levels from TRAPed replicates from the same GFP-rpl10a frog lines as well as from different lines. B: PCA of the contribution of each of the 16 analyzed genes on the overall variance. The placement and length of the vectors show the contribution to the variance for each gene. The gene expression data have been standardized to a value of 1, demarked by the tan circle (see Experimental Procedures section).Download figure to PowerPoint

Image published in: Watson FL et al. (2012)

Copyright © 2012. Image reproduced with permission of the Publisher, John Wiley & Sons.

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