Xenbase Image ID: 78958

RT-PCR analysis in ectodermal explants of embryos injected with chordin and wnt8 RNA. Animal caps were dissected from the embryos injected with 100 pg of chordin (Chd) and/or 200 pg of wnt8 RNA, cultured until stage 18 and subjected to RT-PCR. Neural crest marker slug expression is induced only in the presence of both chordin and wnt8 as previously shown. Only Id3 is maintained under the neural crest-inducing conditions, while Id4 was maintained or up-regulated under neuralizing condition. EF1α expression is used as a loading control. Image published in: Kee Y and Bronner-Fraser M (2005) Copyright © 2005. Image reproduced on with permission of the Publisher, Cold Spring Harbor Laboratory Press. This is an Open Access article. Larger Image Printer Friendly View

Return to previous page