XB-IMG-78988
Xenbase Image ID: 78988
Fig. 5. Double whole-mount in situ analysis of opl.
In situ analysis was as described in Methods with opl stained light blue and all other markers (otx-2, XCG, slug, en-2, krox20) are stained purple.
(A) opl plus otx-2, a marker of forebrain and cement gland (Blitz and Cho, 1995; Pannese et al., 1995), bracket shows otx-2 expression in cement gland primordium;
(B) opl plus XCG, a cement gland marker (Sive et al., 1989), bracket shows XCG expression in cement gland; (C) opl plus slug, a neural crest marker (Mayor et al., 1995), bracket shows slug domain contained within opl domain;
(D) opl plus en-2, a marker of the midbrain/hindbrain regions (Hemmati-Brivanlou and Harland, 1989) and krox20, a marker of presumptive rhombomeres 3 and 5 in the hindbrain (Bradley et al., 1993). White arrowhead indicates en-2 expression, black arrowheads indicate krox20 expression, white bar is posterior limit of early opl expression. Image published in: Kuo JS et al. (1998) Copyright © 1998. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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