Xenbase Image ID: 78990

Fig. 8. oplDC can activate neural crest and dorsal neural tube marker genes in animal caps. (A) Experimental scheme. Wild-type embryos were injected in both blastomeres with 200 pg of indicated RNAs at the 2-cell stage. Injection of CAT RNA served as negative control by equalizing the injected RNA dose. Pools of 15-20 animal caps were isolated from injected embryos at late blastula (stage 9) and incubated in saline until harvest at tailbud (stage 22) for RT-PCR analysis. See Methods for details. (B) Expression of marker genes in animal caps. Uninjected embryos serve as control for baseline expression level. Xash3 (Zimmerman et al., 1993), neurogenin (Ma et al., 1996)and NCAM (Kintner and Melton, 1987) are neuralspecific markers; slug (Mayor et al., 1995) is a neural crest marker; pax3 (Espeseth et al., 1995) marks the dorsal neural tube, shh marks the floorplate (Ekker et al., 1995); XK81 (Jonas et al., 1985) and GATA2 (Walmsley et al., 1994) are markers of the ventral ectoderm; EF1a (Krieg et al., 1989) served as loading control. Samples processed without RT did not show EF1a signal after PCR (not shown). Data from one representative experiment is shown; similar results were obtained from three experiments. Lane 1, stage 22 embryo; lane 2, CAT-injected animal caps; lane 3, oplDC-injected caps; lane 4, opl-injected caps. Image published in: Kuo JS et al. (1998) Copyright © 1998. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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