Xenbase Image ID: 78991

Fig. 9. oplDC sensitizes the ectoderm to induction by noggin. Wildtype embryos were injected in both blastomeres with indicated RNAs at the two-cell stage, using 200 pg globin or oplDC, and 0.1 pg. 1 pg or 10 pg noggin (Smith and Harland, 1992) RNAs, as diagrammed in Fig. 8A, except injection of globin RNA served as negative control by equalizing the injected RNA dose in all lanes. Pools of 15-20 animal caps were isolated from injected embryos at late blastula (stage 9) and incubated in saline until harvest at tailbud (stage 22) for RT-PCR analysis. See Methods for details. Expression of neural markers NCAM (Kintner and Melton, 1987) and Xash3 (Zimmerman et al., 1993) was analysed. EF1a served as loading control (Krieg et al., 1989). Samples processed without RT did not show EF1a signal after PCR (not shown). Data from one representative experiment is shown, similar results were obtained from three experiments. Lane 1, globin-injected caps; lane 2, caps injected with 200 pg oplDC RNA; lane 3, caps injected with 0.1 pg noggin RNA; lane 4, caps injected with 1 pg noggin RNA; lane 5, caps injected with 10 pg noggin RNA; lane 6, caps injected with 0.1 pg noggin RNA plus 200 pg oplDC RNA; lane 7, caps injected with 1 pg noggin RNA plus 200 pg oplDC RNA; lane 8, caps injected with 10 pg noggin RNA plus 200 pg oplDC RNA. Image published in: Kuo JS et al. (1998) Copyright © 1998. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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