Xenbase Image ID: 78993

Fig. 11. opl induces cellular aggregates without cell division. (A) Experimental scheme. Wild-type embryos were injected in one cell at the 2-cell stage with 500 pg of oplGR or oplDC RNA plus 100 pg lacZ RNA as lineage tracer. Injected embryos were incubated in saline alone or with dexamethasone (dex) and/or hydroxyurea plus aphidicolin (HUA) starting at early gastrula (stage 10) until late neurula (stage 19). See Methods for details. (B-H) Morphology and histology of opl-induced cellular aggregates. b-gal staining is obscured by pigment. (B-E) Anterior (A) is to the left, dorsal is to the top. (B) Embryo injected with oplGR RNA without dex addition; (C,D) embryos injected with oplGR RNA and treated with dex from stage 10 until harvest. Arrows point to cellular aggregates in ventrolateral ectoderm. (E) Embryo injected with oplGR RNA and treated with HUA (see Methods) plus dex from stage 10 until harvest. (F-H) Embryos sectioned and stained after oplDC injection. (F) +dex, transverse section of stage 19 embryo through trunk region. Regions magnified in G and H are boxed and indicate uninjected and injected sides of the embryo respectively, as judged by b-gal staining. (G) Uninjected side; (H) injected side. The width of the ectoderm is bracketed in G and H. D, dorsal; V, ventral. Image published in: Kuo JS et al. (1998) Copyright © 1998. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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