Xenbase Image ID: 79011

FIG. 2. E-boxes are dispensable for normal expression of cardiac alpha-actin in embryos, while CArG box 1 and sequence immediately downstream of it are necessary for expression in the heart. (A) Deletion of sequences upstream of -216 results in expression indistinguishable from that obtained with the entire 580-bp pro- moter. (B) 5'delta-103 is expressed in the heart but not in the myotomes. It also gives inappropriate expression in the head. (C) Linker scan mutation LS25 shows no effect on expression. (D) In contrast, LS29, which destroys CArG box 1, results in loss of heart expression. (E) A sequence downstream of CArG box 1 is essential for expression in the heart, as revealed by linker scan mutation LS13. Image published in: Latinkić BV et al. (2002) Copyright © 2002. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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