XB-IMG-79012
Xenbase Image ID: 79012
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FIG. 3. (A) Internal deletion LS10/13 removes CArG boxes 1-4, resulting in weak expression in the myotomes and loss of expression in the heart. (B) An oligonucleotide comprising CArG box 1 and the adjacent 10 bp downstream are sufficient to restore normal expression to LS10/13. (C), L-LS10/13, in which 5 bp most proximal to CArG box 1 are changed, is not expressed in the heart but is expressed normally in the myotomes. (D) Mutation of the more distal 5 bp has no effect on transgene activity in the heart. (E) Mutant T-LS10/13 shows expression in the myotomes, but not in the heart (arrow). (Transgenic constructs shown in B all contain two copies of the oligonucleotides; see Table 1.) Image published in: Latinkić BV et al. (2002) Copyright © 2002. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |