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FIG5. E-boxes mediate silencing of cardiac alpha-actin promoter in serum-stimulated fibroblasts. NIH3T3 fibroblasts were transfected with test plasmids comprising the human c-fos gene driven by the full-length c-fosh promoter (Fos-711) or a chimeric promoter con- taining cardiac actin promoter sequences fused upstream of the c-fos h 5'delta-100. Three cardiac actin promoter regions were tested; the full-length promoter (-580), a 5'truncation that removes the E-box motifs (-246), and an internal deletion of the 580-bp pro- moter that removes 31 bp encompassing the three E-box motifs (deltaM). Cells were serum-starved (-) and then stimulated with serum for 40 min (+). Total RNA was analysed by RNase protections. M-DNA markers; P-undigested riboprobes; C-probes hybridised to tRNA. Diagrams showing essential features of test plas- mids are shown below the gel. Protected Fos and globin transcripts are indicated. Image published in: Latinkić BV et al. (2002) Copyright © 2002. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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