Xenbase Image ID: 79042

Figure 7. Smad5/8 Is Required for Segment Border Formation in Xenopus Embryos. (A) Illustration of C3 Xenopus blastomere injection at the 32 cell stage. The fate of C3 cells in the somites is indicated in a stage 28 tadpole. (B and C) In Xenopus, microinjection of Smad8-MO at the 16 or 32 cell stage (in C2 or C3 blastomeres) erased segmental somite borders on the injected side. Somites are composed mostly of segmental muscles, which were stained for myosin light chain (a-MLC). (D-F0) Smad8-MO effects on segment borders were cell autonomous (co-injection at 32-cell stage with rhodamine dextran amine lineage tracer), and were rescued by human Smad1 mRNA (n = 55, 44 with somite fusions, n = 19, with 17 completely rescued, n = 24, all normal, respectively, 3 independent experiments). Image published in: Eivers E et al. (2009) Eivers et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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