Xenbase Image ID: 79082

Fig. 4. Rfx6 mRNA rescues the morpholino knockdown phenotype. (A) Control whole gut stained for elastase expression (n = 9). (B, C) Whole guts from embryos injected with 40 ng MO2, elastase expression is reduced (n = 47, 6 moderate, 41 severe). (B) Moderate MO2 phenotype, (C) Severe MO2 phenotype. (D) Whole gut from embryo injected with 40 ng MO2 and 100 pg rfx6-GR mRNA. No dexamethosone was added. Elastase expression is reduced (n = 32, 8 normal, 15 moderate, 9 severe). (E) Whole gut from embryo injected with 40 ng MO2 and 100 pg rfx6-GR mRNA. 2 μg/mL dexamethosone was added at NF25 to activate Rfx6-GR protein. Elastase expression is restored (n = 36, 20 normal, 15 moderate, 1 severe). (F) Rescue results from all experimental whole guts. Severe phenotype refers to complete absence of elastase expression and gut malformation; moderate phenotype refers to reduced elastase expression and some gut malformation; normal refers to whole guts with normal levels of elastase expression and normal overall gut morphology. Image published in: Pearl EJ et al. (2011) Copyright © 2011. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue cela1.6 Elas, Elastase, LOC100135144 X. tropicalis Throughout NF stage 42 pancreas Image source: Published Larger Image Printer Friendly View

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