Xenbase Image ID: 79083

Fig. 5. Mutant rfx6 mRNA cannot rescue the morpholino knockdown phenotype. (A) Control whole gut stained for elastase expression (n = 17). (B) Whole gut from embryo injected with 40 ng MO2, elastase expression is reduced (n = 26, 4 normal, 8 moderate, 14 severe). (C) Whole gut from embryo injected with 40 ng MO2 and 100 pg rfx6R181Q-GR mRNA. No dexamethosone was added. Elastase expression is reduced (n = 12, 1 moderate, 11 severe). (D) Whole gut from embryo injected with 40 ng MO2 and 100 pg rfx6R181Q-GR mRNA. 2 μg/mL dexamethosone was added at NF25 to activate Rfx6R181Q-GR protein. Elastase expression remains reduced (n = 16, 3 normal, 4 moderate, 9 severe). (E) Rescue results for three rfx6 mutants: R181Q, S217P, and EX7 (Rfx6 is truncated after exon 7). Severe phenotype refers to complete absence of elastase expression and gut malformation; moderate phenotype refers to reduced elastase expression and some gut malformation; normal refers to whole guts with normal levels of elastase expression and normal overall gut morphology. S217P −dex n = 16, 2 normal, 2 moderate, 14 severe. S217P + dex n = 16, 2 moderate, 14 severe. EX7 −dex n = 13, 1 normal, 1 moderate, 11 severe. EX7 + dex n = 16, 1 normal, 1 moderate, 14 severe. Image published in: Pearl EJ et al. (2011) Copyright © 2011. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue cela1.6 Elas, Elastase, LOC100135144 X. tropicalis Throughout NF stage 42 pancreas Image source: Published Larger Image Printer Friendly View

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