Xenbase Image ID: 79141

Fig. 6. Bicaudal-C and cilia formation. (A–D) Whole mount in situ hybridization of uninjected (A–C) or xBic-C-MO1 + 2-injected Xenopus embryos (D) at stage 36 using (A) FoxJ1, (B) Axonemal Dynein Heavy Chain 9 (ADHC9) and (C, D) polaris. (E–J′) Immunocytochemistry using anti-acetylated α-tubulin antibody (red) on uninjected control, xBic-C-MO1 + 2 and xPol-MO-injected embryos at stage 42. (E, E′, H, H′) Ciliated cells of the epidermis; (F, F′, I, I′) cilia of the nephrostomes; (G, G′, J, J′) individual primary cilia present on the cells of the pronephric duct. (K, K′) Immunocytochemistry using anti-acetylated α-tubulin (red) and anti-NBC1 (green) antibodies on uninjected control and xBic-C-MO1 + 2-injected embryos at stage 42. Images were obtained by confocal microscopy; nuclei were counterstained with DAPI (blue). Image published in: Tran U et al. (2007) Copyright © 2007. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue foxj1.S foxj1-a, foxj1-b, hfh-4, hfh4, xfoxj1 X. laevis Throughout NF stage 35 and 36 pronephric nephrostome ciliated epidermal cell mouth primordium dnah9.S dnah9-A, L-R Dynein, Left-Right Dynein, LRD X. laevis Throughout NF stage 35 and 36 pronephric nephrostome ciliated epidermal cell ift88.S polaris, tg737 X. laevis Throughout NF stage 35 and 36 pronephric nephrostome ciliated epidermal cell tuba4b.L alpha tubulin, alpha-tubulin, tuba4, tuba4a X. laevis Throughout NF stage 42 pronephric duct pronephric nephrostome ciliated epidermal cell epidermis slc4a4.L hhNMC, hnbc1, nbc1, nbc2, nbce1, pNBC, slc4a5, XNBC1 X. laevis Throughout NF stage 42 early proximal tubule Image source: Published Larger Image Printer Friendly View

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